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PH meter for meat monitoring and testing - Project report on quality meat with the help of a pH meter



PH meter for meat monitoring – DIY

Monitoring of meat using a smart pH meter. This project is presented in the following order:
·        Introduction
·         Related literature
·         Components required
·         Blog diagram
·         Principle of operation
·         Circuit diagram


I.                  Introduction
Application: Cold room for monitoring meat to be fit for consumption
The world’s population and that of Cameroon is rapidly increasing and we all know that one of the problems faced, is inadequate supply of food. Meat is one of the most important foods and in some countries it is considered as an essential product with very high consumption rates. In our traditional society, meat (pork, chicken, beef and others) whether fresh or in dry form is an integral part of most meals in homes and restaurants. The quality of these products is of great importance since quality meat is an essential source of nutrients such as: protein, fatty acids, vitamins, minerals and other bioactive compounds which are very important for good health of most of its consumers.

II.               Related literature
The pH value is a widely used way of expressing the acidity or alkalinity of all sorts of products especially in food and is widely used in food processing industry.
PH: pH with full meaning, “pondus hydrogenii (power of hydrogen)” expresses the very small concentrations of hydrogen ions, a measure of alkalinity or acidity of a solution. It was proposed in 1901 by a Danish scientist Soren Peter Lauritz Sorensen.
In 1909 Soren Peter Lauritz Sorensen defined PH as the negative base 10 logarithm of the hydrogen ion activity and derived the equation for calculating the PH.
Where log stands logarithm in base 10 and [H+] stands for the hydrogen ion concentration with units moles per litre.
PH meter
A pH meter is an electrochemical device capable of measuring pH values of acidic and alkaline solutions or samples. The pH is measured on a pH scale of 0 to 14 with a pH of 7 being neutral. A pH less than 7 (pH < 7) is acidic and a pH greater than 7 (pH > 7) is basic or alkaline. Since the pH is measured on a logarithmic scale, the pH value of successive whole number less than 7 is 10 times more acidic than the next one and above 7 it is 10 times more basic than the preceding one.
The meter consists of a pH proton-sensitive electrode usually made of glass and a reference electrode (usually made of silver chloride or calomel) and the electronics of the meter itself.
The figure below shows the pH measurement cell.

Figure 1. A general view of a pH measurement cell
PH of muscle tissue, meat and effects
The pH of muscle tissue is extremely important to meat science since the pH at specific times during the conversion of muscle to meat, as well as the ultimate pH of meat, affects many quality factors.
The ultimate pH is determined by the extent of the pH decline at 24 hours after slaughter. The variation in ultimate pH influences factors such as color and the water retention level of the meat. A low ultimate pH results in meat proteins having decreased water-holding capacity and a lighter color. Conversely, a higher ultimate pH will give a darker color and less drip loss. Other factors include grading characteristics and shrink of carcasses, texture, tenderness, juiciness, taste, appearance.
In a normal living muscle the pH is approximately 7.1-7.2. After slaughtering of the living muscle, some of the glycogen is broken down into lactic acid. Hence, the pH value is lowered. The acidity depends on the type of breed, rearing characteristics and treatment of the animal prior to slaughter. The pH can range from 5.2 to 7.0 with the highest quality in the pH range of 5.7 to 6.0. For example, beef normally reaches its lowest pH value of 5.4 to 5.7 at 18-24 hours after slaughter. After the lowest pH level is reached, the pH starts to rise again slowly but steadily. By the time it reaches pH of 6.5, it starts to decompose. Pork already reaches its lowest pH value of 5.4 to 5.8 at 6-10 hours after slaughter.
Effects of temperature on pH Measurement
Temperature affects pH measurement in two different ways. The first impact of temperature is on the pH electrode itself. It changes its response as temperature also changes. At higher temperatures, they respond more strongly to pH changes. At 100 ºC, they change their output potential by 74 mV/pH unit, and at 0 ºC, they change by 54 mv/pH unit. Because pH meters are typically standardized at pH 7 (that is, zero mv = pH 7), the error from temperature differences gets greater and greater as the pH being measured gets further from 7. So it may be trivial when measuring something with a pH of 7.1, but very important when measuring something with a pH of 10 (or when calibrating with a pH 10 buffer).
Secondly, temperature affects the solution under measurement due to some chemical changes. For example acids can, become stronger or weaker with change in temperature. If the solution is in contact with solids, the temperature can also impact the amount of acid or base is the solution thereby impacting the PH. These effects are specific for every solution you will encounter. 

III.           Components required
·         PIC 16F876A
·         LCD 16X2
·         9V Battery
·         Regulator LM7805
·         Capacitors 1nF(3), 22pF(2)
·         Switch
·         Resistors 10k Ω,470Ω(3), 220Ω
·         Transistor BC547
·         OPAM LM358
·         Analog pH/Moisture sensor
·         5V block relay
·         Temperature sensor DS18B20
·         5kΩ variable resistor
·         Oscillator

IV.           Blog diagram of a pH meter for cold room meat monitoring

Figure 2: Block diagram of the PH meter

V.               Principle of operation
To monitor the meat in a cold room in order to obtain quality meat, the Analog pH/moisture sensor is placed in contact with the meat, the sensor is coupled to an OPAM with the used of a relay which toggles in order to measure the analog values of pH and the moisture of the meat. These analog values are sent to the ADC of the PIC microcontroller which processes the information and displays the corresponding digital values on the LCD. The temperature sensor is also used to monitor the temperature of the meat and displayed on the LCD. LEDs are used to warn if the temperature, pH/Moisture are in or out of range. Hence need for adjustment.
For good storage temperature generally <7oC are acceptable and quality meat should fall within the range:



VI.           Circuit diagram
Simulation of complete diagram with proteus
The complete circuit is shown in the figures below.
Figure 3: complete circuit diagram of the pH meter for meat testing

The first LED1 (D3) indicates the presence of power. LED2 (D4) indicates normal conditions (values are in range). LED3 (D5) is used to indicate abnormal conditions ( the pH/moisture and or the temperature is out of range) , hence need for adjustment.
The simulation of the circuit is shown in the figures below.
Figure 4: Values in range (normal conditions)

The values of the pH, moisture, and temperature are 6.19, 85.60% and 03.750 respectively, which fall within the acceptable range of:
The LED (D4) shines indicating normal conditions.

Figure 5: values out of range (abnormal)

The values of the pH, moisture, and temperature are 4.8, 52.40% and 14.930C respectively, which out of the acceptable range of:
Hence, the LED (D5) shines indicating abnormal conditions. Hence the quality of the meat is not accebtable.

PCB and complete realized circuit



Figure 6. Complete pH meter for meat monitoring

This project can be modified to suit your design contact EKETECH. This can also be modified to suit various applications like in homes, local meat vendor’s areas etc.

Contact for complete circuit file and mikroC code Click here

Reference:
Angong A.N. (2019), pH meter for meat monitoring.
Ek electrical technologies-All about circuits and designs (2019), pH meter for meat monitoring, https://eketechcamer.blogspot.com


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